CRISPR Gene Editing
The Vanderbilt Genome Editing Resource has generated more than 165 mouse strains containing deletions, point mutations, epitope tags, conditional knockouts, fluorescent reporters, recombinases, and more using CRISPR/Cas9.
For more information on specific model types:
- Precise deletions and short insertions
- Large insertions
- piggyBac transgenesis
- CRISPRi
- sgRNA arrays for in vivo CRISPR screening
Full service genome editing projects include:
- Project consultation genome editing projects
- Mutant allele and transgene design
- CRISPR reagent sourcing and quality control
- Embryo microinjection or electroporation
- Screening of founder mice
- Breeding of founders to confirm germline transmission
- Sequencing of N1 heterozygous animals to verify allele fidelity
- Additional quality control assays on a project-specific basis
- Assignment of official MGI allele name and number
- Development of an automated genotyping assay in conjunction with TransnetYX
- Support for manuscripts and grant applications
We can help you test and optimize other types of genome-editing and transgenic technologies under collaborative agreements.