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Vanderbilt Genome Editing Resource

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Vanderbilt Genome Editing Resource (VGER), formerly known as the Transgenic Mouse/ES Cell Shared Resource, has been a trusted and invaluable resource specializing in generating unique mouse lines, cryopreservation, and rederivation for 30 years. Over the years, we have served 340 investigators by generating over 3,500 unique mouse strains. Since 2013, we have produced more than 165 genome-edited alleles using CRISPR/Cas9. In addition to genome editing services, we have also played a crucial role in cryopreserving mouse strains. To date, we have successfully cryopreserved more than 930 unique strains, ensuring the long-term safeguarding of these resources.  Notably, 57 of these preserved lines are now accessible through the Vanderbilt Cryopreserved Mouse Repository.

 

 

IMPACT

  • >200

    CRISPR-edited alleles

  • 20

    sgRNA expression models (CRISPRi)

  • 4

    CRISPR-edited hPSCs

  • 4

    sgRNA array models (iMAP)

  • >1,000

    mouse lines cryopreserved

  • 432

    mouse lines rederived

  • 750

    gene targeted alleles

  • >2700

    transgenic founder mice

  • 83

    strains in the VCMR

Notable publications

Deregulated translation of the transcription factor Myt3 predisposes islet β-cells to dysfunction under obesity-induced metabolic stress.

Hu R, Wang Y, Yagan M, Xu Y, Simmons AJ, Lau KS, Liu Q, Gu G.

Positive autoregulation of Sox17 is necessary for gallbladder and extrahepatic biliary duct formation.

Trinh LT, Finnel RR, Osipovich AB, Musselman JR, Sampson LL, Wright CVE, Magnuson MA.